Additional Selective and Differential Cell Culture Media
Zymo Research Corporation Dual Media Set™ (100 ml EB & 500 ml OB) ^
For cell expansion, E. coli cells are grown in EB™, while the production of the recombinant protein is almost completely repressed. For high level protein expression, the expanded cell culture is simply added into OB™ media. By using the Dual Media Set™ system, protein overexpression can be reliably controlled for many proteins. In some circumstances, such as when expressing toxic proteins, or when the expressed protein is poorly soluble, over-expression is counterproductive. In such cases, limited protein production can be initiated by adding the specific inducer (IPTG for lac-based promoters) to cells growing in EB™.
Bioworld Kao and Michayluk Medium, 25 L
Kao & Michayluk MediumContains Macronutrients, Micronutrients, Vitamins, and Organic Acids as described by Kao & Michayluk (1975) Sucrose, Cytokines, and Auxins should be added as needed Provided as 1 unit to make 10L, 25L or 50L of mediumPlant tissue culture testedWorking Concentration: 3.9 g/LSoluble in water
NEOGEN CLED Medium (Bevis Modification), 500 g
CLED Medium (Bevis Modification) is for the cultivation of coliforms, staphylococci and streptococci, and is not intended for use in the diagnosis of disease or other conditions in humans.Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci. The swarming of Proteus spp. is inhibited. Neogen’s NCM0220 CLED Medium will grow many of the more demanding streptococci of Lancefield groups A, B, C, G and F. This medium may not grow Pasteurella spp. or halophilic organisms.For more information visit https://www.neogen.com/solutions/Microbiology/cled-medium-bevis-modification
NEOGEN Nutrient Agar (BAM), 500 g
Nutrient Agar (BAM) is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar continues to be a widely used general purpose medium for growing non-fastidious microorganisms. If required, enrichments can be added to this medium. Nutrient Agar, modified by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG), is used for fluorogenic detection of Escherichia coli. Nutrient Agar meets APHA and Association of Official Analytical Chemists (AOAC) standard methods. Nutrient Agar is specified in many standard methods procedures for the examination of food, dairy products, water, and other materials.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-agar-bam
Bioworld Agar, Phytoagar, 500g
AgarFree of extra salts that are often present in many agar preparations and affect plant growth. Gel Strength: 550 g/cm2, Working Concentration: 5−7 g/LGelling Temperature: 38 °CMoisture: 10.92%Ash Content: 1.2%Swelling Index: 9.0 mLWater Absorption: 68.0 mLExtraneaous Matter: <0.3%HS Code : 130231000RTECS Number: AW7950000
Sigma Aldrich Fine Chemicals Biosciences Minimum Essential Medium E
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids some at increased concentrations. In addition it contains 5 additional vitamins.
